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Related Experiment Videos

Correlation between cell-adherent activity and surface structure in Porphyromonas gingivalis.

K Watanabe1, Y Yamaji, T Umemoto

  • 1Department of Oral Microbiology, Kanagawa Dental College, Yokosuka, Japan.

Oral Microbiology and Immunology
|December 1, 1992
PubMed
Summary

Porphyromonas gingivalis cell adhesion varies by strain, with hydrophobic strains exhibiting higher binding to human cells. This increased cell adherence is linked to the presence of more fimbriae on the bacterial surface.

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Area of Science:

  • Microbiology
  • Periodontal Disease Research
  • Bacterial Adhesion Mechanisms

Background:

  • Porphyromonas gingivalis is a key pathogen in periodontitis.
  • Understanding bacterial adhesion is crucial for developing targeted therapies.
  • Strain variability in P. gingivalis virulence factors is well-documented.

Purpose of the Study:

  • To compare the cell-adherent ability of six P. gingivalis strains.
  • To investigate the relationship between cell adherence, cell surface hydrophobicity, and fimbriae presence.
  • To identify factors influencing P. gingivalis colonization of host cells.

Main Methods:

  • Radiolabeled P. gingivalis strains were incubated with human gingival fibroblasts, periodontal ligament fibroblasts, and epithelial cells.

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  • Cell-adherent activity was quantified by measuring bound bacterial cells.
  • Bacterial surface hydrophobicity and fimbriae presence were assessed.

    • Main Results:

    • Significant variation in cell-adherent activity was observed among the six P. gingivalis strains.
    • Three strains (381, ATCC 33277, SU63) showed high adherence (14-72%), while three (KD1, W50, W83) showed low adherence (0.6-3.5%).
    • High-adherent strains were hydrophobic and possessed numerous peritrichous fimbriae, whereas low-adherent strains were hydrophilic with few apparent fimbriae.

    Conclusions:

    • P. gingivalis cell-adherent activity is strain-dependent.
    • Bacterial cell surface hydrophobicity and the abundance of fimbriae are key factors in P. gingivalis adherence to human cells.
    • These findings contribute to understanding P. gingivalis pathogenesis and potential therapeutic targets.