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Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
Wild measles virus strain: isolation and identification.
N T Tikhonova1, T A Mamaeva, M A Naumova
1G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russia.
Acta Virologica
|December 1, 1992
Summary
Researchers isolated four measles virus strains from patients and confirmed their identity using cell culture and serological tests. These findings aid in understanding measles virus characteristics and developing diagnostic tools.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Measles virus (MeV) is a significant human pathogen.
- Isolation and characterization of MeV strains are crucial for vaccine development and epidemiological studies.
Purpose of the Study:
- To isolate and identify measles virus strains from patients with active measles.
- To characterize the cytopathic effects and confirm the specificity of the isolated viruses.
Main Methods:
- Virus isolation from mononuclear cells using Vero cell cultures.
- Observation of cytopathic effects, including syncytia formation.
- Hemadsorption assay to confirm viral specificity.
- Virus neutralization (VN) and hemagglutination inhibition (HI) tests for identification.
Main Results:
- Four measles virus isolates (Gag, Il, Buk, Shed) were successfully obtained.
- Infected cell cultures exhibited characteristic syncytia formation.
- Viral specificity was confirmed by syncytia morphology and hemadsorption.
- Isolates were identified using VN and HI tests with standard measles immunoglobulin and hyperimmune sera.
Conclusions:
- The study successfully isolated and identified four measles virus strains.
- The characterized cytopathic effects and serological data confirm the isolates as measles virus.
- These findings contribute to the understanding of measles virus diversity and provide valuable reagents for diagnostic and research purposes.
