Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression during cardiac development.

P J Barton1, P K Bhavsar, N J Brand

  • 1Department of Cardiothoracic Surgery, National Heart and Lung Institute, London.

Symposia of the Society for Experimental Biology
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cardiac malformations, adrenal agenesis, neural crest defects and exencephaly in mice lacking Cited2, a new Tfap2 co-activator.

Nature genetics·2001
Same author

Risk of lymphoid neoplasia after cardiothoracic transplantation. a cohort study of the relation to Epstein-Barr virus.

Transplantation·2001
Same author

Aortic root characteristics of human pulmonary autografts.

Circulation·2000
Same author

Heat shock treatment enhances graft cell survival in skeletal myoblast transplantation to the heart.

Circulation·2000
Same author

Gene therapy for myocardial protection: transfection of donor hearts with heat shock protein 70 gene protects cardiac function against ischemia-reperfusion injury.

Circulation·2000
Same author

Elevated tumor necrosis factor-alpha and interleukin-6 in myocardium and serum of malfunctioning donor hearts.

Circulation·2000
Same journal

The experimental modification of nucleic acid systems in the cell.

Symposia of the Society for Experimental Biology·2010
Same journal

Nucleic acid and the chromosomes.

Symposia of the Society for Experimental Biology·2010
Same journal

The function of deoxyribose-nucleic acid in the cell nucleus.

Symposia of the Society for Experimental Biology·2010
Same journal

The action of enzymes on chromosomes.

Symposia of the Society for Experimental Biology·2010
Same journal

Nucleic acids in the cell and the embryo.

Symposia of the Society for Experimental Biology·2010
Same journal

Histochemical observations on nucleic acids in homologous normal and neoplastic tissues.

Symposia of the Society for Experimental Biology·2010
See all related articles

This study explores embryonic heart development, focusing on cell fate in endocardial cushion tissue and troponin gene expression during fetal myocyte maturation. It also examines identifying transcription factors using polymerase chain reaction cloning techniques.

Area of Science:

  • Developmental Biology
  • Cardiovascular Research
  • Molecular Genetics

Background:

  • Vertebrate heart formation involves myocardial and endocardial layers separated by cardiac jelly.
  • Maturation includes asymmetric cell changes, bulboventricular loop formation, and specialized tissue development.
  • Precise spatial and temporal gene expression control is crucial for cardiac development.

Purpose of the Study:

  • To investigate cell fate determination during embryonic endocardial cushion tissue formation.
  • To analyze troponin gene expression transitions during fetal myocyte maturation.
  • To explore polymerase chain reaction-based cloning for identifying cardiac transcription factors.

Main Methods:

  • Analysis of cell fate determination in embryonic heart development.

Related Experiment Videos

  • Study of troponin gene expression patterns in fetal myocytes.
  • Application of polymerase chain reaction (PCR) cloning techniques.

    • Main Results:

    • Detailed examination of cell fate decisions in the developing endocardial cushion tissue.
    • Characterization of dynamic changes in troponin gene expression during myocyte maturation.
    • Successful identification of cardiac transcription factors using PCR-based cloning.

    Conclusions:

    • Understanding cell fate and gene regulation is key to normal cardiac development.
    • Troponin gene transitions reflect critical stages of fetal myocyte maturation.
    • PCR cloning is a valuable tool for discovering cardiac-specific transcription factors.