[Study on essential metal concentration in the organs of rats (report 2)--lung.liver.kidney.spleen]

The objective of this study was to obtain the normal ranges of essential metals in male and female Slc: Wister rats aged 9 or 11 weeks. Concentration of 10 elements (Ca, Cu, Fe, K, Mg, Mn, Na, P, S and Zn) in the lung, liver, kidney and spleen of rats was determined by inductively coupled argon plasma atomic emission spectrometry (ICP). Each sample was digested with nitric acid and perchloric acid in a sealed double Teflon vessel with polypropylene jacket by heating with a microwave oven. The concentrations of Fe and Mn in the liver were different between males and females. The Fe level in the female was higher than that in the male, and Mn level in male was higher than that in female. The concentration of Fe in the spleen was different by sex and ago. The Fe level in female was 2.3 times higher than that in male, and that in rats of 11 weeks was 1.8 times higher than that of 9 weeks.