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Related Experiment Videos

Bone marrow processing with the Fresenius AS 104: initial results.

J Zingsem1, T Zeiler, R Zimmermann

  • 1Abt. Innere Medizin und Poliklinik m.S. Hämatologie und Onkologie, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, FRG.

Journal of Hematotherapy
|January 1, 1992
PubMed
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This study demonstrates an efficient method for concentrating bone marrow (BM) stem cells using the Fresenius AS 104 cell separator. The protocol yields a high recovery of mononuclear and clonogenic cells, suitable for transplantation.

Area of Science:

  • Hematology
  • Cellular Therapy

Background:

  • Bone marrow (BM) processing is crucial for transplantation.
  • Efficient concentration of BM stem cells is needed for purging, cryopreservation, and transplantation.

Purpose of the Study:

  • To evaluate a modified "grancollect-protocol" and BMSC protocol for BM concentration using the Fresenius AS 104 cell separator.
  • To assess the efficiency and cell recovery of the described BM concentration method.

Main Methods:

  • Concentration of 23 BM harvests using a modified "grancollect-protocol" and BMSC protocol with the Fresenius AS 104 cell separator.
  • Processing initial marrow volumes (mean 922 ml) two to three times within 40-70 minutes.
  • Analysis of mononuclear cell recovery, clonogenic cell recovery (CFU-GM), and red blood cell reduction.

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Main Results:

  • Mean recovery of 59% of initial mononuclear cells in a reduced volume (mean 119 ml).
  • High recovery of clonogenic cells (98% by CFU-GM assays).
  • Significant reduction of red blood cells to 8% of initial numbers.

Conclusions:

  • The described BM concentration procedure is efficient.
  • The method yields a BM cell fraction suitable for purging, cryopreservation, and transplantation.
  • Transplanted patients demonstrated fast and sustained engraftments.