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Effect of aging on macrophage adherence to extracellular matrix proteins
Abstract:
Fibronectin, type I, type IV and type V collagens were compared for their abilities to promote mouse peritoneal resident macrophage adherence and the effect of aging on macrophage adhesion to these matrix proteins was examined. Adherence of macrophages to fibronectin was remarkable and more than 5-fold compared to type I, type IV or type V collagens. Adherence of macrophages to fibronectin and type I collagen increased during aging. However, no age-related changes were observed in macrophage adherence to type IV and type V collagens. The percent of inhibition of macrophage adherence to fibronectin by arginine-glycine-aspartic acid-serine (RGDS) peptide (58.1 +/- 2.7%) was significantly (P < 0.01) higher than that in cells from young mice (23.1 +/- 5.7%). These data suggest that macrophage attach preferentially to fibronectin and that the ability of macrophage to attach to fibronectin increases during aging. The age-related increase in macrophage attachment to fibronectin is related to the concentration of cell surface receptors of macrophage which recognize RGDS sequence within fibronectin during aging. Adherence of macrophage to fibronectin implies retention of macrophages in subendothelial space. Age-related increase in macrophage ability to attach to fibronectin may be related to atherogenesis during aging.
Insights
Macrophage adherence to fibronectin significantly increases with age, unlike other collagens. This age-related increase in fibronectin attachment may contribute to age-related diseases like atherogenesis.
Area of Science:
- Biomedical Science
- Cell Biology
- Immunology
Background:
- Macrophage adherence to extracellular matrix proteins is crucial for immune responses and tissue homeostasis.
- Aging is associated with altered immune cell function and increased susceptibility to diseases such as atherosclerosis.
Purpose of the Study:
- To compare the adhesive properties of fibronectin and various collagen types (I, IV, V) for mouse peritoneal macrophages.
- To investigate the impact of aging on macrophage adherence to these matrix proteins.
- To explore the role of the arginine-glycine-aspartic acid-serine (RGDS) sequence in age-related changes in macrophage adhesion.
Main Methods:
- Mouse peritoneal resident macrophages were isolated.
- Macrophage adherence to immobilized fibronectin, type I, type IV, and type V collagens was quantified.
- The effect of aging on macrophage adherence was assessed.
- Inhibition of macrophage adherence to fibronectin by RGDS peptide was measured.
Main Results:
- Macrophages exhibited significantly higher adherence to fibronectin compared to collagens I, IV, and V (over 5-fold increase).
- Macrophage adherence to fibronectin and type I collagen increased with age.
- No significant age-related changes were observed in macrophage adherence to type IV and type V collagens.
- The inhibition of macrophage adherence to fibronectin by RGDS peptide was significantly higher in aged mice.
Conclusions:
- Macrophages preferentially adhere to fibronectin over certain collagen types.
- Aging enhances macrophage adherence to fibronectin, potentially mediated by increased expression of RGDS-recognizing receptors.
- Increased macrophage attachment to fibronectin during aging may contribute to their retention in the subendothelial space and play a role in atherogenesis.