Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Intramolecular thiolester linkages in apolipoprotein B.

D M Lee¹, S Singh

¹Lipoprotein and Atherosclerosis Research Program, Oklahoma Medical Research Foundation, Oklahoma City 73104.

SAAS Bulletin, Biochemistry and Biotechnology

|January 1, 1990

Summary

This study provides the first evidence of intramolecular thiolester bonds in apolipoprotein B (ApoB). These labile bonds may explain the unusual properties of ApoB and low-density lipoprotein (LDL).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Erratum: Centrality-Dependent Modification of Jet-Production Rates in Deuteron-Gold Collisions at sqrt[s_{NN}]=200 GeV [Phys. Rev. Lett. 116, 122301 (2016)].

Physical review letters·2025

Same author

Trends and projections in sexually transmitted infections in people aged 45 years and older in England: analysis of national surveillance data.

Perspectives in public health·2022

Same author

Salivary Testosterone and Sexual Function and Behavior in Men and Women: Findings from the Third British National Survey of Sexual Attitudes and Lifestyles (Natsal-3).

Journal of sex research·2021

Same author

Image-Guided Transvaginal and Transabdominal Drain Placement for Surgical Navigation in a Patient with Tubo-Ovarian Abscess and Obstructed Hemivagina and Ipsilateral Renal Anomaly Syndrome.

Journal of pediatric and adolescent gynecology·2021

Same author

Purely Spatial Quantum Diffusion of H Atoms in Solid H_{2} at Temperatures below 1 K.

Physical review letters·2021

Same author

Erratum: Evolution of π^{0} Suppression in Au+Au Collisions from sqrt[s_{NN}]=39 to 200 GeV [Phys. Rev. Lett. 109, 152301 (2012)].

Physical review letters·2020

Area of Science:

Biochemistry
Proteomics
Lipid Metabolism

Background:

Apolipoprotein B (ApoB) is a key component of low-density lipoprotein (LDL).
The unusual properties of ApoB and LDL suggest unique structural features.
Intramolecular modifications within ApoB have been hypothesized but not directly demonstrated.

Purpose of the Study:

To investigate the presence and location of intramolecular thiolester bonds in apolipoprotein B (ApoB).
To characterize the chemical nature and potential functional implications of these thiolester linkages.

Main Methods:

Cleavage of thiolester bonds using [14C]methylamine (MA).
Titration of newly generated sulfhydryl groups using [3H]- or [14C]iodoacetate (IA).
Peptide mapping, SDS-PAGE, FPLC, and sequence analysis of labeled ApoB.

Main Results:

Demonstrated covalent incorporation of [14C]MA and [3H]carboxylmethyl groups into ApoB.
Identified two specific labeled peptides containing Cys-51 and Cys-3734.
Provided evidence for thiolester formation between Cys-51/gamma-Glu-54 and Cys-3734/beta-Asp-3737.

Conclusions:

This study presents the first direct evidence for intramolecular thiolester linkages in apolipoprotein B (ApoB).
The identified thiolester bonds involve specific cysteine residues and adjacent amino acids.
The presence of these labile, high-energy bonds may contribute to the unique characteristics of ApoB and LDL.

Related Experiment Videos