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Bioconversions in aqueous two-phase systems.

E Andersson1, B Hahn-Hägerdal

  • 1Department of Applied Microbiology, Lund University, Sweden.

Enzyme and Microbial Technology
|April 1, 1990
PubMed
Summary
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Enzymatic and microbial bioconversions in aqueous two-phase systems offer efficient biocatalyst retention. This review explores partitioning, stability, and process design for improved bioprocesses.

Area of Science:

  • Biotechnology
  • Biochemical Engineering

Background:

  • Bioconversions utilize enzymes or microbial cells for chemical transformations.
  • Aqueous two-phase systems (ATPS) offer unique environments for bioconversions.
  • Efficient biocatalyst recovery and stability are crucial for process economics.

Purpose of the Study:

  • To review bioconversions in ATPS.
  • To discuss the partitioning behavior of biocatalysts, substrates, and products.
  • To summarize biocatalyst retention efficiency and the influence of phase components on biocatalyst performance.

Main Methods:

  • Review of literature on bioconversions in ATPS.
  • Analysis of partitioning principles based on size.
  • Summarization of biocatalyst retention strategies.

Related Experiment Videos

  • Exemplification of phase component effects on enzyme and cell activity.

    • Main Results:

    • Biocatalyst, substrate, and product partitioning in ATPS is size-dependent.
    • ATPS provide efficient biocatalyst retention compared to other methods.
    • Phase components impact enzyme activity (e.g., penicillin acylase, cellulolytic enzymes) and microbial cell performance (e.g., Bacillus sp. for alpha-amylase production).

    Conclusions:

    • Aqueous two-phase systems are effective for biocatalyst immobilization and recycling in bioconversions.
    • Understanding partitioning and phase component effects is key to optimizing ATPS-based bioprocesses.
    • Further considerations for process design and cost-effectiveness are highlighted.