Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein engineering for thermostability.

Y Nosoh1, T Sekiguchi

  • 1Department of Fundamental Science, College of Science and Engineering, Iwaki Meisei University, Fukushima, Japan.

Trends in Biotechnology
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neutron-Multiplicity Measurement in Muon Capture on Oxygen Nuclei in the Gadolinium-Loaded Super-Kamiokande Detector.

Physical review letters·2026
Same author

Results from the T2K Experiment on Neutrino Mixing Including a New Far Detector μ-like Sample.

Physical review letters·2026
Same author

First Differential Measurement of the Single π^{+} Production Cross Section in Neutrino Neutral-Current Scattering.

Physical review letters·2025
Same author

First Measurement of the Electron-Neutrino Charged-Current Pion Production Cross Section on Carbon with the T2K Near Detector.

Physical review letters·2025
Same author

First Joint Oscillation Analysis of Super-Kamiokande Atmospheric and T2K Accelerator Neutrino Data.

Physical review letters·2025
Same author

Search for Periodic Time Variations of the Solar ^{8}B Neutrino Flux between 1996 and 2018 in Super-Kamiokande.

Physical review letters·2024

Amino acid substitutions in small proteins can be predicted, but conformational changes add complexity. Site-directed mutagenesis enhances understanding and production of protein stability.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Engineering

Background:

  • Predicting the impact of amino acid substitutions on protein behavior is crucial in molecular biology.
  • Studies on small, monomeric proteins offer insights but face challenges due to conformational changes.

Purpose of the Study:

  • To explore the predictability of amino acid substitution effects in proteins.
  • To investigate the role of conformational changes in protein stability predictions.
  • To highlight the utility of site-directed mutagenesis in protein engineering.

Main Methods:

  • Utilizing site-directed mutagenesis to introduce specific amino acid substitutions.
  • Analyzing the effects of these substitutions on protein structure and stability.
  • Comparing experimental results with predictive models.

Related Experiment Videos

Main Results:

  • Amino acid substitution effects are partially predictable for small proteins.
  • Conformational dynamics and other factors can complicate accurate predictions.
  • Site-directed mutagenesis facilitates a deeper understanding of protein stability.

Conclusions:

  • Predicting protein stability from sequence modifications is feasible but requires accounting for complex factors.
  • Site-directed mutagenesis is a powerful tool for both understanding protein stability and engineering more stable protein variants.