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Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
Published on: July 29, 2007
An improved method for rapid isolation of plasmid DNA from wild-type gram-negative bacteria for plasmid restriction
1Department of Hygiene and Microbiology, Royal Veterinary and Agricultural University, Frederiksberg C., Denmark.
Letters in Applied Microbiology
|May 1, 1990
Abstract:
An improved method for the isolation of large and small plasmids from wild-type Gram-negative bacteria has been developed. The protocol combines the lysis and purification procedures of two popular plasmid isolation methods, and produces DNA sufficiently pure for restriction enzyme digestion in less than three hours.
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