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Related Experiment Videos

Insect resistant cotton plants.

F J Perlak1, R W Deaton, T A Armstrong

  • 1Monsanto Co., St. Louis, MO 63198.

Bio/Technology (Nature Publishing Company)
|October 1, 1990
PubMed
Summary
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Genetically engineered cotton plants expressing Bacillus thuringiensis insect control proteins effectively protected crops from major lepidopteran pests like the cotton bollworm. This demonstrates significant progress in developing insect-resistant crops for agriculture.

Area of Science:

  • Agricultural Science
  • Biotechnology
  • Entomology

Background:

  • Lepidopteran insect pests cause significant damage to cotton crops, impacting agricultural productivity.
  • Bacillus thuringiensis (B.t.) produces insecticidal proteins effective against certain insect species.

Purpose of the Study:

  • To assess the efficacy of genetically modified cotton expressing truncated insect control protein genes (cryIA(b) and cryIA(c)) from B.t. var. kurstaki.
  • To evaluate insect resistance in cotton plants against key lepidopteran pests under laboratory and field conditions.

Main Methods:

  • Expression of truncated cryIA(b) and cryIA(c) genes in cotton plants.
  • Laboratory assays using Trichoplusia ni (cabbage looper) and Spodoptera exigua (beet armyworm).
  • Field assays with Heliothis zea (cotton bollworm) under high insect pressure.

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  • Immunological analysis to quantify insect control protein levels.

    • Main Results:

    • Cotton plants showed complete protection against leaf damage from T. ni and S. exigua in laboratory tests.
    • Whole plants demonstrated effective protection of squares and bolls against H. zea in field conditions.
    • Insect control proteins constituted 0.05% to 0.1% of total soluble protein in the plants.

    Conclusions:

    • Genetically modified cotton expressing B.t. insect control proteins provides effective resistance against major lepidopteran pests.
    • These findings represent a significant advancement towards the agricultural application of insect-resistant genetically modified crops.