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Insect resistant cotton plants.

F J Perlak¹, R W Deaton, T A Armstrong

¹Monsanto Co., St. Louis, MO 63198.

Bio/Technology (Nature Publishing Company)

|October 1, 1990

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Genetically engineered cotton plants expressing Bacillus thuringiensis insect control proteins effectively protected crops from major lepidopteran pests like the cotton bollworm. This demonstrates significant progress in developing insect-resistant crops for agriculture.

Area of Science:

Agricultural Science
Biotechnology
Entomology

Background:

Lepidopteran insect pests cause significant damage to cotton crops, impacting agricultural productivity.
Bacillus thuringiensis (B.t.) produces insecticidal proteins effective against certain insect species.

Purpose of the Study:

To assess the efficacy of genetically modified cotton expressing truncated insect control protein genes (cryIA(b) and cryIA(c)) from B.t. var. kurstaki.
To evaluate insect resistance in cotton plants against key lepidopteran pests under laboratory and field conditions.

Main Methods:

Expression of truncated cryIA(b) and cryIA(c) genes in cotton plants.
Laboratory assays using Trichoplusia ni (cabbage looper) and Spodoptera exigua (beet armyworm).
Field assays with Heliothis zea (cotton bollworm) under high insect pressure.

Related Experiment Videos

Immunological analysis to quantify insect control protein levels.

Main Results:

Cotton plants showed complete protection against leaf damage from T. ni and S. exigua in laboratory tests.
Whole plants demonstrated effective protection of squares and bolls against H. zea in field conditions.
Insect control proteins constituted 0.05% to 0.1% of total soluble protein in the plants.

Conclusions:

Genetically modified cotton expressing B.t. insect control proteins provides effective resistance against major lepidopteran pests.
These findings represent a significant advancement towards the agricultural application of insect-resistant genetically modified crops.