Related Experiment Videos
Optimal chemostat cascades for periplasmic protein production
R H Davis1, W F Ramirez, A Chatterjee
1Department of Chemical Engineering, University of Colorado, Boulder 80309-0424.
Biotechnology Progress
|November 1, 1990
Summary
This study optimizes protein production using bakers' yeast (Saccharomyces cerevisiae) in a chemostat cascade. A two-stage system offers the highest productivity, with minimal gains over single-stage, especially when using cell recycle.
Area of Science:
- Biotechnology
- Biochemical Engineering
- Microbial Production Systems
Background:
- Optimizing secreted protein production is crucial for industrial applications.
- Bakers' yeast (Saccharomyces cerevisiae) is a common host for protein secretion.
- Protein secretion into the periplasmic space presents unique challenges.
Purpose of the Study:
- To theoretically predict the optimal chemostat cascade design for steady-state protein production.
- To evaluate the impact of cascade staging and cell recycle on productivity.
- To identify design trade-offs for maximizing protein yield.
Main Methods:
- Utilized a theoretical secretion model for Saccharomyces cerevisiae.
- Simulated protein production in single, two, and three-stage chemostat cascades.
- Analyzed the effects of cell concentration and protein production rates with and without cell recycle.
Main Results:
- The highest productivity was achieved with a two-stage chemostat cascade.
- The improvement in productivity from two stages over a single stage was marginal.
- Cell recycle increased both cell concentration and protein productivity.
- Three-stage cascades offered no additional benefit over two-stage systems.
Conclusions:
- A two-stage chemostat cascade represents an optimal design for steady-state secreted protein production in yeast.
- System design trade-offs, particularly with cell recycle, influence the benefits of multi-stage cascades.
- Further optimization can be achieved by considering specific protein production and secretion rates.