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Enzymatic cell lysis for product release.

J A Asenjo¹, B A Andrews

¹University of Reading, England.

Bioprocess Technology

|January 1, 1990

Summary

Lytic enzyme systems offer controlled cell lysis and selective product release, overcoming limitations of mechanical methods for intracellular recombinant proteins. This technology shows promise for large-scale bioprocessing.

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Area of Science:

Biotechnology
Biochemical Engineering
Microbial Cell Lysis

Background:

Mechanical cell disruption lacks specificity and can damage products.
Intracellular recombinant proteins require novel release strategies.
Lytic enzyme systems offer biological specificity for controlled cell lysis.

Purpose of the Study:

Review available lytic enzymes for bacterial and yeast cell lysis.
Discuss mathematical models for lysis process description.
Explore potential for large-scale lytic enzyme technology and differential product release.

Main Methods:

Review of lytic enzymes for bacteria and yeast.
Description of mathematical models (simple, structured, population balance) for lysis.
Discussion of factors affecting enzyme kinetics, recovery, and downstream operations.

Main Results:

Lytic enzyme systems provide specific cell lysis and controlled product release.
Mathematical models aid in understanding and optimizing lysis processes.
Enzyme activity profiles can be manipulated for differential product release.

Conclusions:

Lytic enzyme technology holds significant promise for controlled lysis and selective product recovery.
Process design and optimization are key to maximizing benefits.
Potential for large-scale application in biotechnology.

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