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Separation processes in biotechnology. Aqueous two-phase separations.

P A Albertsson1, G Johansson, F Tjerneld

  • 1University of Lund, Sweden.

Bioprocess Technology
|January 1, 1990
PubMed
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Aqueous two-phase systems efficiently separate biomaterials. Their selectivity and capacity are tunable, making them ideal for large-scale purification of proteins and enzymes.

Area of Science:

  • Biochemistry
  • Separation Science
  • Biotechnology

Background:

  • Aqueous two-phase systems (ATPS) are widely used for separating water-compatible substances.
  • Separation efficiency in ATPS typically correlates with the size of the target molecules or particles.
  • Factors like charge, hydrophobicity, and ligand binding influence partition and separation.

Purpose of the Study:

  • To highlight the utility of aqueous two-phase systems for biomaterial separation.
  • To discuss the factors affecting separation selectivity and capacity in ATPS.
  • To emphasize the suitability of ATPS for large-scale purification of biomaterials.

Main Methods:

  • Utilizing aqueous two-phase systems for partitioning a diverse range of biomaterials.
  • Investigating the impact of molecular size on separation selectivity.

Related Experiment Videos

  • Exploring methods to modulate partition and separation capacity (e.g., charge, hydrophobicity, ligand binding).

    • Main Results:

    • ATPS demonstrate broad applicability for separating substances from peptides to cells.
    • Separation selectivity generally increases with the size of partitioned entities.
    • Partition and separation are effectively influenced by physicochemical properties and specific interactions.

    Conclusions:

    • Aqueous two-phase systems offer a versatile platform for biomaterial separation.
    • The tunable nature of ATPS allows for optimization of purification processes.
    • The operational simplicity and high capacity make ATPS highly suitable for industrial-scale purification of enzymes and proteins.