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Effect of free-cell growth parameters on oxygen concentration profiles in gel-immobilized recombinant Escherichia
J Huang1, C M Hooijmans, C A Briasco
1Laboratoire de Technologie Enzymatique, U.R.A. no. 523 du CNRS, Université de Technologie de Compiègne, France.
Applied Microbiology and Biotechnology
|September 1, 1990
Abstract:
Oxygen concentration profiles in immobilized recombinant cells were measured using a microsensor. Experimental results showed that the final depth of oxygen penetration in the carrageenan gel was always in the range 80-100 microns for the strains and media used, although profiles of oxygen concentration during the early stages of cell growth depended on the strain and nutrient medium used. Variations in the oxygen profiles corresponded to differences in kinetic parameters measured for the same strains and media in free-cell experiments.