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Related Experiment Videos

Bioconversion of avermectin into 27-OH avermectin.

M Chartrain1, R White, R Goegelman

  • 1Merck and Company, Inc., Rahway, NJ 07065.

Journal of Industrial Microbiology
|December 1, 1990
PubMed
Summary

This study optimized the bioconversion of avermectin to its 27-hydroxy derivative using Nocardia autotrophica. Process improvements led to an 8-fold increase in productivity, enhancing avermectin derivative production.

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Area of Science:

  • Microbiology
  • Biotechnology
  • Biochemical Engineering

Background:

  • Avermectin bioconversion to its 27-hydroxy derivative is a key step in producing valuable compounds.
  • Nocardia autotrophica subsp. canberrica is identified as the microbial agent for this transformation.
  • Previous studies may have focused on efficiency, but this work prioritizes productivity.

Purpose of the Study:

  • To enhance the productivity of avermectin bioconversion to its 27-hydroxy derivative.
  • To optimize fermentation conditions for improved yield and scalability.
  • To identify critical process parameters affecting bioconversion.

Main Methods:

  • Fermentation process optimization at shake-flask scale.
  • Systematic examination of physico-chemical parameters including pH, substrate concentration, and C:N ratio.
  • Evaluation of substrate solubilization and addition timing.
  • Scale-up studies assessing antifoam agents and substrate solvents.

Main Results:

  • An 8-fold improvement in bioconversion productivity was achieved through parameter optimization.
  • Key factors influencing yield include pH, substrate concentration, addition time, solubilization, C:N ratio, and medium strength.
  • Lard oil was identified as an effective antifoam agent.
  • Dimethyl sulfoxide (DMSO) replaced the original solvent during scale-up.

Conclusions:

  • Optimized fermentation conditions significantly boost avermectin bioconversion productivity.
  • Process parameters are critical for maximizing the yield of the 27-hydroxy avermectin derivative.
  • The study demonstrates a successful scale-up strategy for industrial application.

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