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Electroporation-induced transformation of Escherichia coli: evaluation of a square waveform pulse
1Division of Biologics, PHLS Centre for Applied Microbiology and Research, Salisbury, Wiltsire, UK.
Letters in Applied Microbiology
|February 1, 1991
Abstract:
Four strains of Escherichia coli were tested for electroporation-induced plasmid transformation using a high voltage pulse in a square waveform. Conditions for optimal transformation were determined for each strain and transformation frequencies found to be comparable to those reported previously for E. coli with a sinusoidal waveform of exponential decaying pulse.