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Enzyme production by recombinant Trichoderma reesei strains.
J M Uusitalo1, K M Nevalainen, A M Harkki
1VTT, Biotechnical Laboratory, Espoo, Finland.
Journal of Biotechnology
|January 1, 1991
Summary
Trichoderma reesei, a fungus, can be genetically engineered to produce specific proteins. This study details using its strong cellobiohydrolase I (CBHI) promoter for enhanced heterologous protein production, like calf chymosin.
Area of Science:
- Biotechnology
- Molecular Biology
- Mycology
Background:
- Trichoderma reesei is a filamentous fungus widely used for industrial enzyme production.
- Genetic engineering has enabled significant improvements in cellulolytic enzyme mixtures.
- The cellobiohydrolase I (CBHI) gene promoter is known for its strength and inducibility.
Purpose of the Study:
- To describe the production of homologous and heterologous proteins using Trichoderma reesei.
- To detail the use of genetic engineering for improved cellulolytic enzyme production.
- To demonstrate the application of the CBHI promoter for heterologous protein expression.
Main Methods:
- Genetic engineering of Trichoderma reesei strains.
- Gene inactivation and promoter modification techniques.
- Utilizing the CBHI promoter for heterologous protein expression.
Main Results:
- Constructed strains secrete novel cellulase mixtures.
- Achieved improvements through gene inactivation and promoter changes.
- Successfully expressed and secreted active calf chymosin using the CBHI promoter.
Conclusions:
- Trichoderma reesei is a versatile host for producing both homologous and heterologous proteins.
- Genetic modifications, particularly promoter utilization, enhance protein production.
- The CBHI promoter is effective for high-level secretion of eukaryotic proteins in Trichoderma.