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Heritable damage to yeast caused by transformation
N Danhash1, D C Gardner, S G Oliver
1Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, UK.
Bio/Technology (Nature Publishing Company)
|February 1, 1991
Abstract:
The introduction of plasmid DNA into yeast by transformation or electroporation, but not by cytoduction, results in the induction of a slow growth phenotype. This phenotype is inherited as a dominant Mendelian trait, which is only exhibited in the absence of the native 2 mu nuclear DNA plasmid of yeast. The use of recombinant DNA technology in yeast, therefore, does not necessarily manipulate the genome in a precise and completely defined way.