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A yeast biosensor for glucose determination.

J Racek1

  • 1Department of Clinical Biochemistry, University Hospital, Plzen, Czechoslovakia.

Applied Microbiology and Biotechnology
|January 1, 1991
PubMed
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A novel yeast biosensor offers a reliable method for glucose determination. This potentiometric biosensor, using Hansenula anomala yeast, provides accurate glucose measurements with a long lifespan.

Area of Science:

  • Biotechnology
  • Electrochemistry
  • Biosensor Technology

Background:

  • Glucose determination is crucial in clinical diagnostics.
  • Existing methods may have limitations in speed, cost, or complexity.
  • Development of novel biosensors is essential for improved glucose monitoring.

Purpose of the Study:

  • To develop and characterize a yeast potentiometric biosensor for glucose determination.
  • To evaluate the performance of the biosensor in terms of response, stability, and interference.
  • To assess the biosensor's applicability for glucose measurement in biological samples like urine.

Main Methods:

  • Immobilization of yeast (Hansenula anomala) cell suspension in calcium alginate gel on a glass electrode surface.
  • Induction of glycolytic enzyme synthesis in yeast prior to immobilization.

Related Experiment Videos

  • Potentiometric measurement of glucose concentration within a specific range (5 x 10(-4)-5 x 10(-3) mol/l).

    • Main Results:

    • The yeast biosensor exhibited a Nernstian response to glucose.
    • Achieved a rapid response time of 5 minutes.
    • Demonstrated a long operational lifetime of at least 2 months.
    • Identified mannose and fructose as the primary interfering substances.
    • Obtained comparable results to a photometric enzymatic method for urine glucose measurement.

    Conclusions:

    • The developed yeast potentiometric biosensor is a viable tool for glucose determination.
    • The biosensor offers advantages in response time and operational stability.
    • It shows potential for practical application in biological fluid analysis, such as urine glucose monitoring.