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A novel synthetic method for hybridoma cell encapsulation
M Carmen Bañó1, S Cohen, K B Visscher
1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139.
Bio/Technology (Nature Publishing Company)
|May 1, 1991
Summary
Hybridoma cells were encapsulated in synthetic polyphosphazene gel microbeads, maintaining viability and antibody production. A poly(L-lysine) membrane enhanced antibody retention, boosting production and showing potential for protein recovery.
Area of Science:
- Biomaterials Science
- Cell Encapsulation Technology
- Biotechnology
Background:
- Hybridoma cells are crucial for monoclonal antibody production.
- Current methods for cell encapsulation can impact cell viability and function.
- Efficient recovery of secreted proteins from encapsulated cells remains a challenge.
Purpose of the Study:
- To develop a novel method for encapsulating hybridoma cells using synthetic polymers.
- To assess the viability and antibody-producing capacity of encapsulated hybridoma cells.
- To create a semipermeable membrane for enhanced antibody retention and recovery.
Main Methods:
- Encapsulation of hybridoma cells in calcium cross-linked polyphosphazene gel microbeads.
- Formation of a semipermeable membrane using poly(L-lysine) on the gel microbeads.
- Monitoring of cell density and antibody production over time.
Main Results:
- Hybridoma cells remained viable and functional after encapsulation.
- Encapsulation within polyphosphazene gels did not impair antibody production.
- The poly(L-lysine) membrane effectively retained antibodies within the beads.
- Cell density increased 3.5-fold and antibody production increased 6.4-fold within 13 days.
Conclusions:
- Simple gelation at room temperature is effective for hybridoma cell encapsulation.
- Synthetic polyphosphazene gels support hybridoma cell viability and antibody secretion.
- The developed semipermeable membrane technology shows promise for improved protein recovery strategies.