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High performance capillary electrophoresis.
1Department of Medicinal and Analytical Chemistry, Genentech, Inc., South San Francisco, CA 94080.
Trends in Biotechnology
|July 1, 1991
Summary
Recombinant-DNA technology spurs new analytical methods for biological molecules. High performance capillary electrophoresis (HPCE) offers high-resolution separation, potentially rivaling high performance liquid chromatography (HPLC).
Area of Science:
- Biochemistry
- Analytical Chemistry
- Biotechnology
Background:
- Recombinant-DNA technology has advanced therapeutic protein production over the last decade.
- This advancement necessitates new technologies for analyzing and characterizing biological molecules.
- High performance capillary electrophoresis (HPCE) is gaining attention in scientific communities.
Purpose of the Study:
- To highlight the emergence of High Performance Capillary Electrophoresis (HPCE) as a key analytical technique.
- To discuss the potential of HPCE in the analysis and characterization of biological molecules.
- To compare HPCE with existing separation techniques like High Performance Liquid Chromatography (HPLC).
Main Methods:
- Review of technological advancements in biological molecule analysis.
- Discussion of the principles and applications of High Performance Capillary Electrophoresis (HPCE).
- Comparative analysis of HPCE and High Performance Liquid Chromatography (HPLC) in terms of efficiency and scope.
Main Results:
- HPCE is identified as an extremely high-resolution separation technique.
- HPCE shows potential to rival HPLC in efficiency and application breadth.
- Significant interest in HPCE is observed among biochemists, analytical chemists, and chromatographers.
Conclusions:
- HPCE represents a significant advancement in the analysis of biological molecules.
- The technique offers high efficiency and broad applicability, comparable to HPLC.
- HPCE is poised to become a vital tool in biochemical and analytical laboratories.