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Antibody production in baculovirus-infected insect cells
J zu Putlitz1, W L Kubasek, M Duchêne
1Laboratorium für molekulare Biologie-Genzentrum-der Ludwig-Maximilians-Universität München, Martinsried, FRG.
Bio/Technology (Nature Publishing Company)
|July 1, 1990
Abstract:
We have employed the baculovirus expression system for the production of a mouse monoclonal IgG antibody directed against lipoprotein I of Pseudomonas aeruginosa. Both light and heavy chain cDNAs were introduced into the baculovirus genome in a single step of homologous recombination. Insect cells that were infected with the recombinant virus stably secreted antigen-binding and glycosylated antibody molecules capable of binding the complement component C1q.