Related Experiment Videos
High efficiency electroporation of Lactococcus lactis subsp. lactis LM0230 with plasmid pGB301
1Department of Food and Agricultural Microbiology, Queen's University of Belfast, Northern Ireland.
Letters in Applied Microbiology
|August 1, 1990
Abstract:
Electroporation-mediated transformation of Lactococcus lactis with plasmid pGB301, a 9.8 kilobase pair vector (Behnke et al. 1981), has been reported by McIntyre & Harlander (1989a). Improved transformation efficiencies of 10(2)-10(3)/micrograms DNA were achieved by altering the conditions under which the bacteria were grown prior to electroporation (McIntyre & Harlander 1989b). This present investigation sought to improve still further transformation efficiencies in order to provide a reliable high frequency transformation system for Lc. lactis subsp. lactis.