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Improvement of glidobactin A production by Polyangium brachysporum
1Industrial Division, Bristol-Myers Squibb Co., Syracuse, NY 13221.
Abstract:
Glidobactins A, B, and C are lipopeptide antitumor antibiotics produced by the gliding bacterium Polyangium brachysporum sp. nov. No. K481-B101. The production of glidobactin A was examined in shake flasks and laboratory fermentors. Medium screening and optimization led to approximately five fold increases in glidobactin A titers in shake flasks and a ten fold increase in titers in 40-l batch fermentations. Utilization of a stepped glucose feeding protocol resulted in glidobactin A titers of 1860 micrograms/ml after 144 h of fermentation.
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