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Updated: Jul 11, 2026

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast
Published on: March 24, 2010
Heterologous gene expression in continuous cultures of budding yeast
D Porro1, E Martegani, B M Ranzi
1Dipartimento di Fisiologia e Biochimica generali, Università di Milano, Italy.
Abstract:
In a previous paper we have studied the expression of beta-galactosidase from Escherichia coli, driven from the inducible GAL1-10/CYC1 hybrid promoter, in batch cultures of budding Saccharomyces cerevisiae and have described operating conditions for maximal productivity. In this paper we show that the plasmid instability in continuous cultures can be overcome by utilizing appropriate selection markers and a high copy number vector. The maximal level of expression is influenced by the dilution rate. Moreover, enzyme accumulation appears to depend also upon the degree of oxygenation. A possible explanation of these modulations is discussed, taking into account the interactions of the UAS-GAL and TATA-CYC1 elements.
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