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Modular integrated fluidized bed bioreactor technology.

M Reiter1, G Blüml, T Gaida

  • 1Institute of Applied Microbiology (IAM), University of Agriculture, Vienna, Austria.

Bio/Technology (Nature Publishing Company)
|November 1, 1991
PubMed
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A novel modular bioreactor system uses an internal impeller for recirculation, enabling efficient cell culture. This integrated fluidized bed bioreactor achieved a 100-fold increase in Chinese Hamster Ovary (CHO-K1) cell density.

Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Cell Culture Technology

Background:

  • Traditional fluidized bed bioreactors often rely on external recirculation loops.
  • These systems can be complex and may introduce shear stress, impacting cell viability.
  • There is a need for integrated, modular bioreactor designs offering flexibility and improved performance.

Purpose of the Study:

  • To design and demonstrate a modular integrated fluidized bed bioreactor system.
  • To incorporate an internal low shear stress impeller for recirculation, replacing external loops.
  • To showcase the system's versatility and high cell density cultivation capabilities.

Main Methods:

  • The system features a reactor vessel with an extending cylinder and liquid distributor.

Related Experiment Videos

  • A low shear stress impeller is integrated for internal liquid recirculation.
  • Modular elements allow for operation as a stirred tank, packed bed, or fluidized bed reactor.

    • Main Results:

    • The bioreactor system demonstrated modular exchangeable elements and efficient oxygenation.
    • Operation as a fluidized bed reactor using Chinese Hamster Ovary (CHO-K1) cells was successful.
    • A 100-fold increase in cell density was achieved, reaching up to 1.2 x 10(8) cells/ml.

    Conclusions:

    • The developed modular integrated fluidized bed bioreactor offers a versatile and efficient platform for cell culture.
    • The internal impeller design minimizes shear stress and simplifies the system.
    • The system facilitates high cell density cultivation, showing significant potential for bioprocessing applications.