Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Affinity chromatography for protein isolation.

W H Scouten1

  • 1Baylor University, Texas.

Current Opinion in Biotechnology
|February 1, 1991
PubMed
Summary
This summary is machine-generated.

New affinity chromatography techniques enhance protein purification. Key advancements include immobilized membrane affinity chromatography, temperature-programmed elution, and centrifugal affinity chromatography for diverse applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Affinity purification of natural ligands.

Current protocols in protein science·2008
Same author

Boronate affinity chromatography.

Methods in molecular biology (Clifton, N.J.)·2000
Same author

Alginate as a displacer for protein displacement chromatography.

Journal of molecular recognition : JMR·1996
Same author

Studies on oriented and reversible immobilization of glycoprotein using novel boronate affinity gel.

Journal of molecular recognition : JMR·1996
Same author

Dihydrofolate reductase synthesis in the presence of immobilized methotrexate. An approach to a continuous cell-free protein synthesis system.

Journal of molecular recognition : JMR·1996
Same author

Oriented immobilization of restriction endonuclease EcoRI.

Journal of molecular recognition : JMR·1996
Same journal

Microbial C1 assimilation pathways for chemical synthesis: from native metabolism to synthetic design.

Current opinion in biotechnology·2026
Same journal

Medicinal plants fermentation: current knowledge and perspectives.

Current opinion in biotechnology·2026
Same journal

Fermented foods: lessons learned from metagenomics.

Current opinion in biotechnology·2026
Same journal

Microfluidic platforms for the transient transfection of mammalian cells: recent developments and challenges.

Current opinion in biotechnology·2026
Same journal

Harvesting insights from recent advances in yeast genomics for predictable and precision wine fermentation.

Current opinion in biotechnology·2026
Same journal

Minimal enzyme cascades for the aromatic-to-aromatic upgrading of lignin monomers.

Current opinion in biotechnology·2026
See all related articles

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Separation Science

Background:

  • Protein purification is crucial in biological research and biotechnology.
  • Affinity chromatography is a widely used technique for protein purification.
  • The field of protein purification is mature, with continuous innovation.

Purpose of the Study:

  • To highlight significant advancements in affinity chromatography techniques.
  • To review recent applications of affinity chromatography in protein purification.
  • To identify emerging trends in the field.

Main Methods:

  • Review of over 150 reports on protein purification involving affinity chromatography.
  • Focus on novel techniques such as immobilized membrane affinity chromatography.

Related Experiment Videos

  • Analysis of temperature-programmed elution and centrifugal affinity chromatography.

    • Main Results:

    • Affinity chromatography remains a central technique in protein purification.
    • Immobilized membrane affinity chromatography offers new possibilities.
    • Temperature-programmed elution and centrifugal affinity chromatography represent significant progress.

    Conclusions:

    • The field of affinity chromatography is dynamic, with ongoing development of innovative techniques.
    • These new methods expand the scope and efficiency of protein purification.
    • Continued research in affinity chromatography is essential for advancing biological sciences.