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Related Experiment Videos

Expression cloning systems.

A Aruffo1

  • 1Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington.

Current Opinion in Biotechnology
|October 1, 1991
PubMed
Summary
This summary is machine-generated.

Expression cloning in Xenopus oocytes and mammalian cells is highly effective for isolating cDNA clones. Recent advances in screening methodologies enable the isolation of cDNAs encoding intracellular proteins.

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Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • Expression cloning is a powerful technique for identifying genes based on the function of the proteins they encode.
  • Various expression systems exist, including Xenopus oocytes, fission yeast, and mammalian cells, each with unique advantages and limitations.

Purpose of the Study:

  • To review and compare the utility of different expression cloning systems.
  • To highlight the effectiveness and versatility of Xenopus oocytes and mammalian cells for expression cloning.
  • To discuss recent advancements in screening methodologies for isolating intracellular protein-encoding cDNAs.

Main Methods:

  • Review of literature on expression cloning techniques.
  • Comparison of transient expression systems in Xenopus oocytes and mammalian cells.

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  • Analysis of recent screening methodology advancements.

    • Main Results:

    • Transient expression cloning in Xenopus oocytes and mammalian cells are the most effective and versatile systems.
    • A large number of cDNA clones have been isolated using these two methods.
    • Advances in screening have enabled the isolation of cDNAs encoding intracellular proteins.

    Conclusions:

    • Xenopus oocytes and mammalian cells are superior systems for expression cloning.
    • New screening techniques are expanding the scope of expression cloning to intracellular proteins.