Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzyme stabilization strategies based on electrolytes and polyelectrolytes for biosensor applications.

Nikolas A Chaniotakis1

  • 1Laboratory of Analytical Chemistry, Department of Chemistry, University of Crete, 71 409 Iraklion, Crete, Greece. nchan@chemistry.uoc.gr

Analytical and Bioanalytical Chemistry
|September 19, 2003
PubMed
Summary

Stabilizers like electrolytes, polyelectrolytes, and polyols significantly enhance enzyme stability in biosensors. These compounds protect enzymes from degradation, improving both shelf life and performance for future biosensor applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thick membrane, solid contact ion selective electrode for the detection of lead at picomolar levels.

Analytical chemistry·2005
Same author

Carbon nanotube array-based biosensor.

Analytical and bioanalytical chemistry·2003
See all related articles

Area of Science:

  • Biochemistry and Materials Science
  • Focuses on enzyme stabilization techniques and their application in biosensor development.

Background:

  • Enzyme deactivation in biosensors is a major challenge, caused by thermal shock, proteolysis, and oxidation.
  • Current enzyme stabilization methods show limited application in biosensor technology.

Purpose of the Study:

  • To review achievements in enzyme stabilization using electrolytes, polyelectrolytes, and polyols.
  • To evaluate the impact of these stabilizers on biosensor storage and operational stability.

Main Methods:

  • Review of existing literature on enzyme stabilization techniques.
  • Analysis of stabilizer effects on enzyme deactivation pathways (thermal, proteolytic, oxidative).
  • Deconvolution of stabilizer impacts on storage versus operational stability.

Related Experiment Videos

Main Results:

  • Electrolytes, polyelectrolytes, and polyols can improve both storage and operational stability of biosensors.
  • These stabilizers effectively reduce enzyme deactivation from thermal shock, proteolytic degradation, and metal-catalyzed oxidation.
  • The application of established enzyme stabilization techniques to biosensors is currently limited.

Conclusions:

  • Enzyme stabilization using electrolytes, polyelectrolytes, and polyols offers significant potential for improving biosensor performance.
  • Wider adoption of these stabilization techniques is expected to drastically enhance biosensor storage and operational stabilities.
  • Further research and application are needed to fully leverage existing enzyme stabilization methods in biosensor technology.