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Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Updated: Jun 5, 2026

Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Peptide screening.

S Birnbaum¹, K Mosbach

¹Department of Pure and Applied Biochemistry, University of Lund, Sweden.

Current Opinion in Biotechnology

|February 1, 1992

Summary

This summary is machine-generated.

Advances in peptide preparation and screening since the 1990s have enabled the production of diverse peptide libraries. New methods utilize phage display and chemical synthesis for expanded peptide discovery.

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Peptide-based Identification of Functional Motifs and their Binding Partners

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Area of Science:

Biochemistry and Molecular Biology
Synthetic Chemistry

Background:

Significant advancements in peptide preparation and screening technologies have emerged since the late 1990s.
The demand for large and diverse peptide libraries has driven innovation in synthesis and selection methodologies.

Purpose of the Study:

To review and highlight key developments in peptide preparation and screening.
To discuss the impact of new technologies on peptide library generation and application.

Main Methods:

Phage display technology for peptide library screening.
Chemical synthetic strategies including light-directed, spatially addressable synthesis.
Single-bead, single-peptide synthesis and iterative selection/synthesis approaches.

Main Results:

Development of robust methods for generating and screening vast peptide libraries.
Increased efficiency and diversity in peptide library construction.
Facilitation of peptide discovery for various applications.

Conclusions:

Modern peptide preparation and screening techniques have revolutionized the field.
These advancements provide powerful tools for discovering novel peptides with desired functions.