Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Robust microsatellite instability (MSI) analysis by denaturing high-performance liquid chromatography (DHPLC).

Il-Jin Kim1, Yong Shin1, Hio Chung Kang1

  • 1Korean Hereditary Tumor Registry, Cancer Research Institute and Cancer Research Center, Seoul National University, Seoul, Korea.

Journal of Human Genetics
|September 19, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ESRP1-regulated DNMT3B isoform switching determines the malignant potential of pancreatic cancer.

Oncogene·2026
Same author

Establishment, biobanking, and multi-omics characterization of 41 patient-derived colorectal cancer organoids: MYC/PRC classification.

Translational oncology·2026
Same author

Bacteriophage-mediated restoration of epithelial integrity, DNA damage response, and transcriptomic homeostasis in colon organoids exposed to ETBF and pks + E. coli.

Scientific reports·2026
Same author

Ferroptosis-based therapeutic strategy targeting iron metabolism and SLC7A11 overcomes oxaliplatin resistance in patient-derived pancreatic cancer organoids.

Journal of experimental & clinical cancer research : CR·2026
Same author

A spatially resolved human glioblastoma atlas reveals distinct cellular and molecular patterns of anatomical niches.

Nature communications·2026
Same author

Pressurized intraperitoneal aerosol chemotherapy enhances cisplatin efficacy in colorectal cancer organoids.

Scientific reports·2025

A new, rapid denaturing high-performance liquid chromatography (DHPLC) method accurately analyzes microsatellite instability (MSI) in colorectal cancers. This efficient technique offers a reliable alternative for MSI detection, crucial for cancer diagnosis and prognosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Microsatellite instability (MSI) is a significant biomarker in various cancers, particularly colorectal cancer.
  • Accurate and efficient MSI analysis is crucial for cancer diagnosis, prognosis, and treatment selection.

Purpose of the Study:

  • To develop a simple, efficient, and robust method for microsatellite instability (MSI) analysis.
  • To evaluate the utility of denaturing high-performance liquid chromatography (DHPLC) for MSI detection in colorectal cancer.

Main Methods:

  • DNA was extracted from 175 colorectal cancer samples and matched normal tissues.
  • Five Bethesda microsatellite markers were analyzed for MSI using denaturing high-performance liquid chromatography (DHPLC) at 50°C and 0.9 ml/min flow rate.

Related Experiment Videos

  • DHPLC results were validated using conventional gel electrophoresis and capillary sequencing.

    • Main Results:

    • The DHPLC method determined MSI status in just 9 minutes per PCR product.
    • Out of 175 samples, 45 (26%) were high MSI (MSI-H), 12 (7%) were low MSI (MSI-L), and 118 (67%) were microsatellite stable (MSS).
    • MSI was significantly associated with proximal colon cancers (p<0.0001), TNM stage I/II (p=0.05), and high-grade tumors (p<0.01).

    Conclusions:

    • A DHPLC-based method provides a rapid, simple, and efficient alternative for robust MSI analysis.
    • This method can aid in the accurate detection and characterization of MSI in colorectal cancer.
    • The findings support the clinical relevance of MSI in specific colorectal cancer subtypes and stages.