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Microbial modification of sugars as building blocks for chemicals
E Stoppok1, K Matalla, K Buchholz
1Institut fur landwirtschaftliche Technologie und Zuckerindustrie an der TU Braunschweig, Langer Kamp 5, Germany.
Applied Microbiology and Biotechnology
|February 1, 1992
Summary
Agrobacterium tumefaciens NCPPB 396 efficiently oxidizes sucrose to a 3-keto-derivative. Optimal microbial modification depends on reaction time, aeration, and substrate concentration for maximum yield.
Area of Science:
- Microbiology
- Biochemistry
- Enzymology
Background:
- Microbial transformations offer specific and efficient synthesis routes.
- Agrobacterium tumefaciens is known for its metabolic capabilities.
Purpose of the Study:
- To investigate the microbial oxidation of sucrose to its 3-keto-derivative using resting cells of Agrobacterium tumefaciens NCPPB 396.
- To kinetically analyze the factors influencing the production of the 3-keto-derivative.
Main Methods:
- Utilized resting cells of Agrobacterium tumefaciens NCPPB 396 for sucrose oxidation.
- Performed kinetic analysis by varying substrate concentration, cell mass, reaction time, and aeration rates.
Main Results:
- The microbial oxidation is highly specific for the 3-keto-derivative.
- Product formation is significantly influenced by reaction time and aeration rate, with optimal rates up to 11.5 volume air/cultivation volume per minute.
- Product yield increases with substrate concentration, but is maximized below 20 g/l.
Conclusions:
- Agrobacterium tumefaciens NCPPB 396 is a suitable microorganism for producing sucrose 3-keto-derivatives.
- Optimized reaction conditions (aeration, substrate concentration) are crucial for maximizing yield.
- The study provides data on active cell mass production and derivative formation, with potential applications.