Molecular characterization of two host-guest associating hyaluronan derivatives
Related Experiment Video
Updated: Sep 13, 2026

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion
Published on: June 15, 2019
1Institute of Experimental Pharmacology, Slovak Academy of Sciences, SK-84104 Bratislava, Slovak Republic. exfasolt@savba.sk
Abstract:
Molecular characteristics were determined of two high-molecular-weight water-soluble hyaluronan derivatives, namely beta-cyclodextrin (HA-beta-CD) and N-acylurea (EDC-HA). The weight-average molecular weight (M(w)) of HA-beta-CD and of EDC-HA, determined with a multi-angle light scattering detector connected on-line to a size exclusion chromatographic system, was respectively 185.3 and 86.8 kDa. However the M(w) value determined for the equimolar mixture of the two HA derivatives equaled 556.0 kDa. Similarly, the gyration radius of the above equimolar mixture, Rg = 80.6 nm, was significantly greater than the values found for the single HA derivative, i.e. 40.2 nm for HA-beta-CD and 23.8 nm for EDC-HA. These data indicate that the two kinds of substituents, bound to the polymeric chains, form host-guest/inclusion complexes resulting in polymacromolecular associates/aggregates.
Related Concept Videos
Glycosaminoglycans
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...
Proteoglycans
Glycocalyx and its Functions
Components of...
Oligosaccharide Assembly
Multiple sugar molecules that may or may...

