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Related Experiment Videos

A low cost microprocessor-controlled electrofusion and electroporation system.

C A McCormick1, M O Toll, W H Marshall

  • 1Faculty of Engineering, Memorial University of Newfoundland, St John's, Canada.

Journal of Chemical Technology and Biotechnology (Oxford, Oxfordshire : 1986)
|January 1, 1992
PubMed
Summary
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Researchers developed an affordable electrofusion device using a microcomputer for electroporation and electrofusion. This system enables cost-effective gene transfer and hybridoma cell production for monoclonal antibody research.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Bioinstrumentation

Background:

  • Electroporation and electrofusion use high electric fields to alter cell membranes.
  • These techniques are crucial for gene transfer and creating hybrid cells, such as hybridomas for monoclonal antibody production.
  • The high cost of commercial electrofusion equipment limits research accessibility.

Purpose of the Study:

  • To design and construct an inexpensive electrofusion device.
  • To provide a cost-effective alternative to commercially available electrofusion systems.
  • To enable researchers to assemble a flexible and affordable instrument.

Main Methods:

  • A Radio Shack CoCo III microcomputer programmed in BASIC controls the system.
  • The device delivers controlled electroporation voltage pulses (25-500 V, 2-275 µs) to an electrofusion chamber.

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  • The system achieves electric field strengths of 0.25-5 kV cm⁻¹ with pulse currents up to 3 A.

    • Main Results:

    • An inexpensive electrofusion system was successfully assembled for under $800 Canadian.
    • The constructed instrument offers greater flexibility compared to existing commercial options.
    • The system effectively controls electroporation parameters for cell manipulation.

    Conclusions:

    • The developed electrofusion device provides a low-cost, high-flexibility solution for biotechnology research.
    • This accessible instrument facilitates gene transfer and hybridoma cell production.
    • The technical details enable replication, reducing barriers to advanced cell manipulation techniques.