Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Culture01:21

Cell Culture

23.1K
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
23.1K
Cell Lines01:16

Cell Lines

10.5K
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
10.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The application status, challenges and prospects of artificial intelligence in communicable diseases prevention and control of health facilities in China].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2025
Same author

[Epidemic characteristics and dynamic changes of spatio-temporal distribution of hemorrhagic fever with renal syndrome in Guangzhou, 2010-2019].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2020
Same author

Methods to manufacture regulatory T cells for cell therapy.

Clinical and experimental immunology·2019
Same author

Maximizing the security of chaotic optical communications.

Optics express·2016
Same author

Comparing maternal and perinatal outcomes in pregnancies complicated by preeclampsia superimposed chronic hypertension and preeclampsia alone.

Clinical and experimental obstetrics & gynecology·2016
Same author

Increased titer and reduced lactate accumulation in recombinant retrovirus production through the down-regulation of HIF1 and PDK.

Biotechnology and bioengineering·2015
Same journal

Microbial C1 assimilation pathways for chemical synthesis: from native metabolism to synthetic design.

Current opinion in biotechnology·2026
Same journal

Medicinal plants fermentation: current knowledge and perspectives.

Current opinion in biotechnology·2026
Same journal

Fermented foods: lessons learned from metagenomics.

Current opinion in biotechnology·2026
Same journal

Microfluidic platforms for the transient transfection of mammalian cells: recent developments and challenges.

Current opinion in biotechnology·2026
Same journal

Harvesting insights from recent advances in yeast genomics for predictable and precision wine fermentation.

Current opinion in biotechnology·2026
Same journal

Minimal enzyme cascades for the aromatic-to-aromatic upgrading of lignin monomers.

Current opinion in biotechnology·2026
See all related articles

Related Experiment Video

Updated: Feb 20, 2026

Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids
11:01

Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids

Published on: September 25, 2016

8.2K

Mammalian cell culture processes.

W S Hu1, J M Piret

  • 1Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis 55455.

Current Opinion in Biotechnology
|April 1, 1992
PubMed
Summary
This summary is machine-generated.

Researchers are exploring how culture conditions impact mammalian cell viability, productivity, and protein modifications. Advances in bioreactor technology and fed-batch strategies aim to optimize recombinant protein production.

More Related Videos

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
08:39

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Published on: December 5, 2011

38.5K
Trypsinizing and Subculturing Mammalian Cells
05:59

Trypsinizing and Subculturing Mammalian Cells

Published on: June 12, 2008

22.3K

Related Experiment Videos

Last Updated: Feb 20, 2026

Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids
11:01

Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids

Published on: September 25, 2016

8.2K
Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
08:39

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Published on: December 5, 2011

38.5K
Trypsinizing and Subculturing Mammalian Cells
05:59

Trypsinizing and Subculturing Mammalian Cells

Published on: June 12, 2008

22.3K

Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Cell Biology

Background:

  • Mammalian cell culture is crucial for producing complex biologics.
  • Optimizing culture conditions is key to improving cell viability and productivity.
  • Ensuring consistency in post-translational modifications is vital for therapeutic efficacy.

Purpose of the Study:

  • Investigate the influence of culture conditions on mammalian cell viability and productivity.
  • Enhance the consistency of post-translational modifications in recombinant proteins.
  • Develop advanced fed-batch strategies for optimizing protein production processes.

Main Methods:

  • Utilizing novel biosensor and bioreactor developments.
  • Applying new technologies to study high cell density bioreactor conditions.
  • Developing kinetic models to understand medium condition effects.

Main Results:

  • Identified key culture parameters affecting cell viability and productivity.
  • Demonstrated improved consistency in post-translational modifications.
  • Advanced understanding of fed-batch strategies for enhanced protein yield.

Conclusions:

  • Culture condition optimization is essential for efficient mammalian cell-based protein production.
  • Novel technologies are enabling better control and understanding of bioreactor processes.
  • Fed-batch strategies coupled with advanced monitoring show promise for industrial applications.