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Improving purification of recombinant ribonuclease T1
O Landt1, M Zirpel-Giesebrecht, A Milde
1Abteilung Saenger, Institut für Kristallographie, Freie Universität Berlin, Germany.
Journal of Biotechnology
|June 1, 1992
Abstract:
Purification of recombinant RNase T1 and its mutants has been improved by optimizing bacterial growth conditions, periplasmic fraction preparation and the use of a precolumn. The main part of the chromatographic separation could be automated due to the reproducibility of the procedure.