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RAPD analysis of Campylobacter isolates: DNA fingerprinting without the need to purify DNA
S Mazurier1, A van de Giessen, K Heuvelman
1Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Abstract:
A method was developed to obtain reproducible DNA fingerprints from Campylobacter by PCR-based amplification, without the need to isolate total DNA. Randomly amplified polymorphic DNA (RAPD) profiles were generated with three randomly designed 10-mers, using each separately as an amplification primer. A range of C. jejuni serotypes could be typed by RAPD analysis. Depending on the primer, the analysis of RAPD profiles resulted in different levels of discrimination between the strains. Clear correlations were observed between results of RAPD analysis and serotyping. Two of the primers tested generated RAPD profiles which allowed discrimination of strains within given Penner and Lior serotypes.