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Fusion proteins in biotechnology.

M Uhlén1, G Forsberg, T Moks

  • 1Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm, Sweden.

Current Opinion in Biotechnology
|August 1, 1992
PubMed
Summary

Gene fusion technology enables creating novel recombinant proteins by combining parental gene traits. These versatile techniques now find applications in protein refolding, immunology, and drug targeting.

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Area of Science:

  • Molecular Biology
  • Protein Engineering
  • Biotechnology

Background:

  • Gene fusion techniques combine genetic material from different genes.
  • Initially used for studying gene expression and protein localization.
  • Facilitated protein purification and analysis.

Purpose of the Study:

  • To review the evolution and applications of gene fusion techniques.
  • To highlight emerging uses in various biological and medical fields.
  • To provide an overview of recombinant protein production via gene fusion.

Main Methods:

  • Review of existing literature on gene fusion applications.
  • Analysis of historical and recent developments in the field.
  • Synthesis of information on protein refolding, immunology, and drug targeting.

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Main Results:

  • Gene fusions have expanded beyond basic research applications.
  • New applications include protein refolding, immunology, drug targeting, and protein display.
  • The versatility of gene fusion technology has significantly increased.

Conclusions:

  • Gene fusion techniques are a powerful tool in molecular biology and biotechnology.
  • The adaptability of gene fusion allows for innovation in protein engineering and therapeutic development.
  • Continued exploration of gene fusion applications promises further advancements.