Related Experiment Videos
FAB-MS/MS spectrometry in determining the primary structure of gamma-glutamyl-containing peptides
1Laboratory of Organic Chemistry, School of Agriculture, Nagoya University, Japan.
Agricultural and Biological Chemistry
|July 1, 1990
Abstract:
Positive fast atom bombardment tandem mass spectrometry (FAB-MS/MS) was applied for peptide sequencing, particularly for determining the gamma glutamyl linkage involved in metal-binding peptides such as Cadystin (gamma EC)3G = Cadystin A and Cadystin (gamma EC)2G = Cadystin B (MW 771 and 539, respectively). The fragmentation patterns between the natural gamma glutamyl peptide and the synthetic alpha glutamyl one were clearly distinguishable. FAB-MS/MS was proved to be a good method for determining these peptides, since it needed no chemical degradation and only a small amount of the peptide was needed for determination.