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Serum-inducible gene expression in fibroblasts

R R Hirschhorn1

  • 1School of Biological Sciences, University of Kentucky, Lexington 40506-0225.

SAAS Bulletin, Biochemistry and Biotechnology
|January 1, 1992
PubMed

Insights

Researchers identified annexin II, a gene involved in cell growth regulation. Its expression changes with cell stimulation, suggesting a role in the cell proliferation process.

Area of Science:

  • Cell biology
  • Molecular biology
  • Biochemistry

Background:

  • Mammalian cell proliferation is controlled by external growth factors.
  • Biochemical pathways triggered by growth factors modulate gene expression, leading to cell growth.
  • Identifying genes that regulate cell growth is crucial for understanding cell proliferation.

Purpose of the Study:

  • To identify, isolate, and characterize genes that modulate mammalian cell growth.
  • To determine the functions of the protein products of these growth-regulated genes.
  • To investigate the role of annexin II in the mitogenic process.

Main Methods:

  • Sequence analysis to identify growth-regulated genes.
  • Monitoring cytoplasmic transcript induction upon serum stimulation of quiescent cells.
  • Analyzing changes in gene expression and protein localization in response to mitogenic stimulation.

Main Results:

  • Several growth-regulated genes were identified, with induced cytoplasmic transcripts upon serum stimulation.
  • Annexin II was identified as a growth-regulated gene and a major substrate of tyrosine kinases.
  • Annexin II expression is growth-regulated, and its association with the membrane/cytoskeleton changes during mitogenic stimulation.

Conclusions:

  • Growth-regulated genes, including annexin II, are functionally involved in the mitogenic process.
  • Annexin II plays a role in regulating cell proliferation.
  • Changes in annexin II's cellular localization correlate with cell growth stimulation.

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