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Large-scale purification and preliminary structural studies of MAC 182, a gibberellin-binding monoclonal antibody
J E Nester-Hudson1, M H Beale, J MacMillan
1Department of Agricultural Sciences, University of Bristol, AFRC Institute of Arable Crops Research, U.K.
Phytochemistry
|October 1, 1992
Abstract:
The large-scale purification of the anti-gibberellin monoclonal antibody, MAC 182, is described. N-Terminal amino acid sequences of the heavy and light chains were determined and compared with those of known antibodies. Fab-fragments were prepared and purified to a state suitable for crystallization.