Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein export in Escherichia coli.

K Johnson1, C K Murphy, J Beckwith

  • 1Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.

Current Opinion in Biotechnology
|October 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Observations on the persistence and vertical transmission of Salmonella enterica serovars Pullorum and Gallinarum in chickens: effect of bacterial and host genetic background.

Avian pathology : journal of the W.V.P.A·2009
Same author

Further studies on vertical transmission and persistence of Salmonella enterica serovar Enteritidis phage type 4 in chickens.

Avian pathology : journal of the W.V.P.A·2009
Same author

Effect of enrofloxacin administration on excretion of Salmonella ententidis by experimentally infected chickens and on quinolone resistance of their Escherichia coli flora.

Avian pathology : journal of the W.V.P.A·2008
Same author

Functional activity of eukaryotic signal sequences in Escherichia coli: the ovalbumin family of serine protease inhibitors.

Journal of molecular biology·2003
Same author

The transmembrane domain of the DnaJ-like protein DjlA is a dimerisation domain.

Molecular genetics and genomics : MGG·2003
Same author

Racism: a central problem for the Human Genome Diversity Project.

Politics and the life sciences : the journal of the Association for Politics and the Life Sciences·2003

Protein export in Escherichia coli is crucial for cell function. This study uses multiple techniques to detail the protein translocation process and the roles of key cellular components.

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • Protein export is essential for bacterial viability and function.
  • The translocation of proteins across the cell envelope is a complex process.
  • Escherichia coli serves as a model organism for studying protein export.

Purpose of the Study:

  • To elucidate the mechanisms of protein export in Escherichia coli.
  • To identify and characterize the cellular components involved in protein translocation.
  • To present novel findings on the function of these components.

Main Methods:

  • Genetic analysis to identify key genes and mutations.
  • Biochemical assays to study protein interactions and enzyme activity.
  • Biophysical techniques to analyze protein structure and dynamics.

Related Experiment Videos

Main Results:

  • Detailed characterization of the protein export pathway.
  • Identification of essential cellular components for protein translocation.
  • New insights into the functional roles of specific export machinery components.

Conclusions:

  • The study provides a comprehensive understanding of protein export in E. coli.
  • The identified components and pathways are critical for bacterial survival.
  • Further research on these components may lead to novel therapeutic targets.