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A novel and efficient method for enzymatic synthesis of high purity maltose using moranoline (1-deoxynojirimycin)
S Maruo1, H Yamashita, K Miyazaki
1Chemistry Laboratories, Nippon Shinyaku Co., Ltd., Kyoto, Japan.
Bioscience, Biotechnology, and Biochemistry
|September 1, 1992
Abstract:
A transglycosylation reaction with moranoline (1-deoxynojirimycin) was done with soluble starch as the glucosyl donor and Bacillus macerans amylase as a cyclodextrin glycosyltransferase [EC 2.4.1.19]. The resultant transglycosylation products with moranoline, obtained by treating the reaction mixture with a strong cation exchange resin, were hydrolyzed by beta-amylase [EC 3.2.1.2] from sweet potatoes. The hydrolysate was treated with a strong cation exchange resin, and high purity maltose was obtained.