Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recombinant human insulin.

M R Ladisch1, K L Kohlmann

  • 1Laboratory of Renewable Resources Engineering, A. A. Potter Engineering Center, Purdue University, West Lafayette, Indiana 47907.

Biotechnology Progress
|November 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Liquefaction of sugarcane bagasse for enzyme production.

Bioresource technology·2014
Same author

Evaluation of a kinetic model for computer simulation of growth and fermentation by Scheffersomyces (Pichia) stipitis fed D-xylose.

Biotechnology and bioengineering·2014
Same author

Patents and literature.

Applied biochemistry and biotechnology·2013
Same author

Solid-state fermentation of lignocellulosic plant residues from Brassica napus by Pleurotus ostreatus.

Applied biochemistry and biotechnology·2004
Same author

Expression of cellular antigens of Listeria monocytogenes that react with monoclonal antibodies C11E9 and EM-7G1 under acid-, salt- or temperature-induced stress environments.

Journal of applied microbiology·2003
Same author

Characterization of dicarboxylic acids for cellulose hydrolysis.

Biotechnology progress·2001
Same journal

Purification and concentration of model viruses using single-pass tangential flow filtration.

Biotechnology progress·2026
Same journal

Advanced glucose control strategies leveraging Raman spectroscopy for optimized mammalian cell culture manufacturing.

Biotechnology progress·2026
Same journal

Mechanistic deconvolution of BSA size variants by constrained Raman pseudo-Voigt hard modeling during anion-exchange chromatography.

Biotechnology progress·2026
Same journal

Status and future of recombinant adeno-associated virus vector manufacturing.

Biotechnology progress·2026
Same journal

Multifaceted algae as an ingredient in alternative meat formulations.

Biotechnology progress·2026
Same journal

In-line Raman spectroscopy real-time glucose prediction method for commercial pneumococcal vaccine drug substance fermentation manufacturing process control.

Biotechnology progress·2026
See all related articles

Recombinant DNA technology enables insulin production for therapeutic use. This review covers insulin manufacturing, detection, and purification methods, including chromatography, for enhanced quality control.

Area of Science:

  • Biotechnology
  • Pharmaceutical Science
  • Analytical Chemistry

Background:

  • Insulin is a crucial peptide hormone for glucose regulation.
  • Traditional insulin production involves pancreatic extraction.
  • Recombinant DNA technology offers an alternative for large-scale insulin synthesis.

Purpose of the Study:

  • To provide an overview of insulin production methods.
  • To review insulin detection and separation techniques.
  • To discuss the advantages and disadvantages of various purification strategies.

Main Methods:

  • Overview of traditional mammalian pancreatic extraction.
  • Description of recombinant production in bacterial and yeast systems.
  • Review of detection techniques including electrophoresis.

Related Experiment Videos

  • Analysis of separation methods: ion exchange, reversed-phase, and size exclusion chromatography.

    • Main Results:

    • Recombinant DNA technology is a viable method for producing therapeutic insulin.
    • Reversed-phase chromatography is key for resolving insulin variants like desamido insulin and proinsulin.
    • Multistep chromatographic processes are essential for process-scale insulin purification.

    Conclusions:

    • Recombinant insulin production offers advantages for therapeutic applications.
    • Advanced chromatographic techniques are critical for ensuring insulin purity and safety.
    • Understanding separation principles aids in optimizing insulin manufacturing processes.