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Related Experiment Videos

Affinity chromatography supports: a look at performance requirements.

S R Narayanan1, L J Crane

  • 1Research and Development Laboratories, J. T. Baker Inc., Phillipsburg, New Jersey 08865.

Trends in Biotechnology
|January 1, 1990
PubMed
Summary

Affinity chromatography offers high selectivity for purifying therapeutic proteins and peptides. This review explores current affinity supports and optimization strategies for large-scale biomolecule purification.

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Area of Science:

  • Biochemistry
  • Chemical Engineering
  • Biotechnology

Background:

  • Affinity chromatography is a key technique in downstream processing for high-value therapeutic proteins and peptides.
  • Its high selectivity makes it ideal for purifying complex biomolecules.

Purpose of the Study:

  • To review available affinity supports for biomolecule purification.
  • To investigate performance characteristics and properties of support matrices for improved large-scale purification.
  • To highlight optimization parameters and advantages of affinity chromatography for scaled-up systems.

Main Methods:

  • Literature review of current affinity chromatography supports.
  • Analysis of performance characteristics and required properties of support matrices.

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  • Examination of process optimization parameters and scaled-up system advantages.

    • Main Results:

    • Identification of various affinity supports and their suitability for different biomolecules.
    • Discussion of critical properties for effective support matrices in large-scale applications.
    • Elucidation of key parameters for optimizing affinity chromatographic processes.

    Conclusions:

    • Affinity chromatography is a powerful tool for large-scale purification of therapeutic biomolecules.
    • Careful selection of affinity supports and process optimization are crucial for efficient purification.
    • The review provides insights into advancing affinity-based separation technologies for biopharmaceutical manufacturing.