Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Overloaded hollow-fiber liquid chromatography.

H B Ding1, E L Cussler

  • 1Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis 55455.

Biotechnology Progress
|November 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[A preliminary study on the short-term effectiveness and safety of sublingual immunotherapy-spray for patients with respiratory allergy].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2024
Same author

[HIV self-testing reagent use in pre-exposure prophylaxis and related factors in men who have sex with men].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2021
Same author

[Factors related to antiretroviral therapy among HIV/AIDS positive students aged 15-30, in a hospital of Shenyang city].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2017
Same author

Cyanide recovery across hollow fiber gas membranes.

Environmental science & technology·2011
Same author

Self-assembled block copolymer thin films as water filtration membranes.

ACS applied materials & interfaces·2010
Same author

Enhancement of food waste digestion in the hybrid anaerobic solid-liquid system.

Water science and technology : a journal of the International Association on Water Pollution Research·2008
Same journal

Advanced glucose control strategies leveraging Raman spectroscopy for optimized mammalian cell culture manufacturing.

Biotechnology progress·2026
Same journal

Mechanistic deconvolution of BSA size variants by constrained Raman pseudo-Voigt hard modeling during anion-exchange chromatography.

Biotechnology progress·2026
Same journal

Status and future of recombinant adeno-associated virus vector manufacturing.

Biotechnology progress·2026
Same journal

Multifaceted algae as an ingredient in alternative meat formulations.

Biotechnology progress·2026
Same journal

In-line Raman spectroscopy real-time glucose prediction method for commercial pneumococcal vaccine drug substance fermentation manufacturing process control.

Biotechnology progress·2026
Same journal

Prolonged autophagy induction correlates with host cell protein reduction in CHO cell culture.

Biotechnology progress·2026
See all related articles

Hollow fiber liquid chromatography offers efficient solute separation with lower pressure drops. Performance predictability varies with flow and feed concentration, impacting flavor and protein separation dynamics.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Biotechnology

Background:

  • Liquid chromatography in hollow fibers utilizes an organic solvent stationary phase for solute separation.
  • This technique offers significantly lower pressure drops compared to traditional packed beds.

Purpose of the Study:

  • To investigate the chromatographic separation of solutes, including flavors and proteins, using hollow fiber systems.
  • To evaluate the influence of flow rates and feed concentrations on separation efficiency and predictability.

Main Methods:

  • Employing hollow fiber liquid chromatography with an organic solvent stationary phase.
  • Analyzing solute dispersion and correlating results with dimensionless parameters like the Graetz number and dimensionless flux.

Related Experiment Videos

Main Results:

  • Separations demonstrated dispersion consistent with chromatographic theories at low flows and dilute feeds.
  • Performance became less predictable at high flows and concentrated feeds, indicating hollow fiber overload.
  • Flavor separation correlated well with the Graetz number, aligning with adsorption theories.
  • Protein separation showed poor correlation with the Graetz number but better correlation with a dimensionless flux based on facilitated diffusion.

Conclusions:

  • Hollow fiber chromatography is effective for separating flavors and proteins, with predictable performance under specific conditions.
  • The separation behavior of proteins is better described by facilitated diffusion models than general chromatographic theories at higher concentrations.