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Overloaded hollow-fiber liquid chromatography.
1Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis 55455.
Biotechnology Progress
|November 1, 1990
Summary
Hollow fiber liquid chromatography offers efficient solute separation with lower pressure drops. Performance predictability varies with flow and feed concentration, impacting flavor and protein separation dynamics.
Area of Science:
- Analytical Chemistry
- Separation Science
- Biotechnology
Background:
- Liquid chromatography in hollow fibers utilizes an organic solvent stationary phase for solute separation.
- This technique offers significantly lower pressure drops compared to traditional packed beds.
Purpose of the Study:
- To investigate the chromatographic separation of solutes, including flavors and proteins, using hollow fiber systems.
- To evaluate the influence of flow rates and feed concentrations on separation efficiency and predictability.
Main Methods:
- Employing hollow fiber liquid chromatography with an organic solvent stationary phase.
- Analyzing solute dispersion and correlating results with dimensionless parameters like the Graetz number and dimensionless flux.
Main Results:
- Separations demonstrated dispersion consistent with chromatographic theories at low flows and dilute feeds.
- Performance became less predictable at high flows and concentrated feeds, indicating hollow fiber overload.
- Flavor separation correlated well with the Graetz number, aligning with adsorption theories.
- Protein separation showed poor correlation with the Graetz number but better correlation with a dimensionless flux based on facilitated diffusion.
Conclusions:
- Hollow fiber chromatography is effective for separating flavors and proteins, with predictable performance under specific conditions.
- The separation behavior of proteins is better described by facilitated diffusion models than general chromatographic theories at higher concentrations.