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Related Experiment Videos

High-resolution particle size analysis in biotechnology process control.

J C Thomas1, A P Middelberg, J F Hamel

  • 1Brookhaven Instruments Corporation, Holtsville, New York.

Biotechnology Progress
|July 1, 1991
PubMed
Summary
This summary is machine-generated.

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Optimizing centrifuge feed rates using particle size distribution measurements improves the recovery of protein inclusion bodies. This method enhances production efficiency for industrially important proteins like porcine somatotropin.

Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Protein Production

Background:

  • Industrially significant proteins are often produced as insoluble intracellular protein inclusion bodies.
  • Large-scale centrifugation is crucial for separating and recovering these inclusion bodies during production.
  • Optimizing centrifuge feed rate is essential for maximizing recovery efficiency and minimizing production costs.

Purpose of the Study:

  • To investigate the use of a disc centrifuge photosedimentometer for monitoring protein inclusion body recovery.
  • To measure the particle size distribution (PSD) of the supernatant during porcine somatotropin (pST) inclusion body production.
  • To enable optimization of centrifugation operations for improved inclusion body recovery.

Main Methods:

Related Experiment Videos

  • Utilized a disc centrifuge photosedimentometer for high-resolution particle size distribution (PSD) measurements.
  • Monitored the supernatant during the production of porcine somatotropin (pST) inclusion bodies.
  • Analyzed PSD data to detect the breakthrough of inclusion bodies into the supernatant.

    • Main Results:

    • High-resolution PSD measurements effectively monitored inclusion body breakthrough.
    • The method allowed for real-time assessment of centrifugation performance.
    • Data obtained facilitated the optimization of centrifuge feed rates.

    Conclusions:

    • Disc centrifuge photosedimentometry is a valuable tool for monitoring inclusion body recovery.
    • Optimized centrifugation parameters, guided by PSD analysis, can enhance protein production efficiency.
    • This approach offers a practical solution for improving the recovery of industrially relevant protein inclusion bodies.